In some situations it may become necessary to make sure that BizTalk is not caching anything. If one suspects that a bindings configuration is being cached, this is the directory to look for. (This is for BizTalk 2010, Windows Server 2008)
C:\Users\{YourLogin}\AppData\Roaming\Microsoft\BizTalk Server\Deployment\BindingFiles
(Replace {YourLogin} with the login used to deploy the application(s) in question.)
The files in this directory can be deleted, and BizTalk would no longer have any cache of your bindings configuration